TitleComparison of methods for the isolation and culture of Migratory chondroprogenitors from Human articular cartilage.
Publication TypeJournal Article
Year of Publication2023
AuthorsVinod E, Parasuraman G, Livingston A, Amirtham SManickam, Rebekah G, J Lisha J, Daniel AJob, Sathishkumar S
JournalConnect Tissue Res
Date Published2023 Jul
KeywordsCartilage, Articular, Cell Differentiation, Cells, Cultured, Chondrocytes, Chondrogenesis, Humans, Hypertrophy, Stem Cells

PURPOSE: Resident articular stem cells isolated using a migratory assay called Migratory Chondroprogenitors (MCPs) have emerged as a promising cellular therapeutic for the treatment of cartilage pathologies. In-vivo studies using MCPs report their superiority over bone-marrow mesenchymal stem cells and chondrocytes for treating chondral defects. However, there is no consensus on their isolation protocol. This study aimed to compare four reported isolation methods of MCPs and identify the optimal and feasible protocol for future translational work.

METHODS: Human MCPs isolated from osteoarthritic cartilage ( = 3) were divided into four groups: a) MCP1: 8-15 mm cartilage explants, b) MCP2: 8-10 mm explants digested in 0.1% collagenase for 2 hrs. and cultured c) MCP3: 1 mm cartilage explants and d) MCP 4: 25 mm explants with a X tear, 7-day culture, and trypsinization to release migrated cells. The MCPs were subjected to the following analysis: growth kinetics, surface marker expression, mRNA gene expression for markers of chondrogenesis and hypertrophy, and trilineage differentiation.

RESULTS: MCPs isolated via the four methods showed similar surface marker profiles, chondrogenic (SOX-9, ACAN, COL2A1) and hypertrophic (COL1, RUNX2) gene expression. The migration time for the MCP3 group was the longest. The MCP1, MCP2, and MCP4 groups produced MCPs with comparable cellular expansion feasibility.

CONCLUSIONS: MCPs can be preferably isolated by the any of the three above methods based on the investigator's discretion. In the case of small cartilage samples similar to the MCP3 group, the isolation of MCP is plausible, keeping in mind the additional time required.

Alternate JournalConnect Tissue Res
PubMed ID37092666