TitleComprehensive Analysis of microRNAs in Human Adult Erythropoiesis.
Publication TypeJournal Article
Year of Publication2021
AuthorsNath A, Rayabaram J, Ijee S, Bagchi A, Chaudhury ADutta, Roy D, Chambayil K, Singh J, Nakamura Y, Velayudhan SR
Date Published2021 Nov 04
KeywordsAdult, Cell Differentiation, Cell Line, Chromatin, CRISPR-Cas Systems, Erythroid Cells, Erythropoiesis, Gene Editing, Gene Expression Profiling, Gene Expression Regulation, Humans, MicroRNAs, Signal Transduction, Transcription Factors

MicroRNAs (miRNAs) are small non-coding RNAs, which play an important role in various cellular and developmental processes. The study of miRNAs in erythropoiesis is crucial to uncover the cellular pathways that are modulated during the different stages of erythroid differentiation. Using erythroid cells derived from human CD34+ hematopoietic stem and progenitor cells (HSPCs)and small RNA sequencing, our study unravels the various miRNAs involved in critical cellular pathways in erythroid maturation. We analyzed the occupancy of erythroid transcription factors and chromatin accessibility in the promoter and enhancer regions of the differentially expressed miRNAs to integrate miRNAs in the transcriptional circuitry of erythropoiesis. Analysis of the targets of the differentially expressed miRNAs revealed novel pathways in erythroid differentiation. Finally, we described the application of Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) based editing of miRNAs to study their function in human erythropoiesis.

Alternate JournalCells
PubMed ID34831239
PubMed Central IDPMC8616439
Grant ListBT/PR17316/MED/31/326/2015 / / Department of Biotechnology, Govt. of India. /