@article {3343,
	title = {Initiation of wound healing is regulated by the convergence of mechanical and epigenetic cues.},
	journal = {PLoS Biol},
	volume = {20},
	year = {2022},
	month = {2022 09},
	pages = {e3001777},
	abstract = {<p>Wound healing in the skin is a complex physiological process that is a product of a cell state transition from homeostasis to repair. Mechanical cues are increasingly being recognized as important regulators of cellular reprogramming, but the mechanism by which it is translated to changes in gene expression and ultimately cellular behavior remains largely a mystery. To probe the molecular underpinnings of this phenomenon further, we used the down-regulation of caspase-8 as a biomarker of a cell entering the wound healing program. We found that the wound-induced release of tension within the epidermis leads to the alteration of gene expression via the nuclear translocation of the DNA methyltransferase 3A (DNMT3a). This enzyme then methylates promoters of genes that are known to be down-regulated in response to wound stimuli as well as potentially novel players in the repair program. Overall, these findings illuminate the convergence of mechanical and epigenetic signaling modules that are important regulators of the transcriptome landscape required to initiate the tissue repair process in the differentiated layers of the epidermis.</p>
},
	keywords = {Biomarkers, Caspase 8, Cues, Epigenesis, Genetic, Wound Healing},
	issn = {1545-7885},
	doi = {10.1371/journal.pbio.3001777},
	author = {Bhatt, Tanay and Dey, Rakesh and Hegde, Akshay and Ketkar, Alhad Ashok and Pulianmackal, Ajai J and Deb, Ashim P and Rampalli, Shravanti and Jamora, Colin}
}
@article {1578,
	title = {Interactions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells.},
	journal = {Methods Mol Biol},
	volume = {1879},
	year = {2019},
	month = {2019},
	pages = {285-297},
	abstract = {<p>The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk comes the requirement of methods to interrogate the molecular underpinnings of these leukocyte-stem cell interactions. Here we describe how a combination of FACS, cellular coculture assays, and conditioned media treatments can be utilized to advance our understanding of this emerging area of intercellular communication between immune cells and stem cells.</p>
},
	issn = {1940-6029},
	doi = {10.1007/7651_2018_155},
	author = {Badarinath, Krithika and Dutta, Abhik and Hegde, Akshay and Pincha, Neha and Gund, Rupali and Jamora, Colin}
}
