TY - JOUR T1 - Mitochondrial state determines functionally divergent stem cell population in planaria. JF - Stem Cell Reports Y1 - 2021 A1 - Mohamed Haroon, Mohamed A1 - Lakshmanan, Vairavan A1 - Sarkar, Souradeep R A1 - Lei, Kai A1 - Vemula, Praveen Kumar A1 - Palakodeti, Dasaradhi AB -

Mitochondrial state changes were shown to be critical for stem cell function. However, variation in the mitochondrial content in stem cells and the implication, if any, on differentiation is poorly understood. Here, using cellular and molecular studies, we show that the planarian pluripotent stem cells (PSCs) have low mitochondrial mass compared with their progenitors. Transplantation experiments provided functional validation that neoblasts with low mitochondrial mass are the true PSCs. Further, the mitochondrial mass correlated with OxPhos and inhibiting the transition to OxPhos dependent metabolism in cultured cells resulted in higher PSCs. In summary, we show that low mitochondrial mass is a hallmark of PSCs in planaria and provide a mechanism to isolate live, functionally active, PSCs from different cell cycle stages (G0/G1 and S, G2/M). Our study demonstrates that the change in mitochondrial metabolism, a feature of PSCs is conserved in planaria and highlights its role in organismal regeneration.

VL - 16 IS - 5 ER -