TY - JOUR T1 - Biophysical properties of the isolated spike protein binding helix of human ACE2. JF - Biophys J Y1 - 2021 A1 - Das, Anirban A1 - Vishvakarma, Vicky A1 - Dey, Arpan A1 - Dey, Simli A1 - Gupta, Ankur A1 - Das, Mitradip A1 - Vishwakarma, Krishna Kant A1 - Roy, Debsankar Saha A1 - Yadav, Swati A1 - Kesarwani, Shubham A1 - Venkatramani, Ravindra A1 - Maiti, Sudipta KW - Angiotensin-Converting Enzyme 2 KW - COVID-19 KW - Humans KW - Peptidyl-Dipeptidase A KW - Protein Binding KW - SARS-CoV-2 KW - Spike Glycoprotein, Coronavirus AB -

The entry of the severe acute respiratory syndrome coronavirus 2 virus in human cells is mediated by the binding of its surface spike protein to the human angiotensin-converting enzyme 2 (ACE2) receptor. A 23-residue long helical segment (SBP1) at the binding interface of human ACE2 interacts with viral spike protein and therefore has generated considerable interest as a recognition element for virus detection. Unfortunately, emerging reports indicate that the affinity of SBP1 to the receptor-binding domain of the spike protein is much lower than that of the ACE2 receptor itself. Here, we examine the biophysical properties of SBP1 to reveal factors leading to its low affinity for the spike protein. Whereas SBP1 shows good solubility (solubility > 0.8 mM), circular dichroism spectroscopy shows that it is mostly disordered with some antiparallel β-sheet content and no helicity. The helicity is substantial (>20%) only upon adding high concentrations (≥20% v/v) of 2,2,2-trifluoroethanol, a helix promoter. Fluorescence correlation spectroscopy and single-molecule photobleaching studies show that the peptide oligomerizes at concentrations >50 nM. We hypothesized that mutating the hydrophobic residues (F28, F32, and F40) of SBP1, which do not directly interact with the spike protein, to alanine would reduce peptide oligomerization without affecting its spike binding affinity. Whereas the mutant peptide (SBP1) shows substantially reduced oligomerization propensity, it does not show improved helicity. Our study shows that the failure of efforts, so far, to produce a short SBP1 mimic with a high affinity for the spike protein is not only due to the lack of helicity but is also due to the heretofore unrecognized problem of oligomerization.

VL - 120 IS - 14 ER -