TY - JOUR T1 - Proteome plasticity in response to persistent environmental change. JF - Mol Cell Y1 - 2021 A1 - Domnauer, Matthew A1 - Zheng, Fan A1 - Li, Liying A1 - Zhang, Yanxiao A1 - Chang, Catherine E A1 - Unruh, Jay R A1 - Conkright-Fincham, Juliana A1 - McCroskey, Scott A1 - Florens, Laurence A1 - Zhang, Ying A1 - Seidel, Christopher A1 - Fong, Benjamin A1 - Schilling, Birgit A1 - Sharma, Rishi A1 - Ramanathan, Arvind A1 - Si, Kausik A1 - Zhou, Chuankai KW - Acclimatization KW - Adaptation, Physiological KW - Animals KW - Environmental Exposure KW - Gene Expression Regulation, Fungal KW - Hot Temperature KW - Proteome KW - Saccharomycetales KW - Stress, Physiological KW - Transcriptome AB -

Temperature is a variable component of the environment, and all organisms must deal with or adapt to temperature change. Acute temperature change activates cellular stress responses, resulting in refolding or removal of damaged proteins. However, how organisms adapt to long-term temperature change remains largely unexplored. Here we report that budding yeast responds to long-term high temperature challenge by switching from chaperone induction to reduction of temperature-sensitive proteins and re-localizing a portion of its proteome. Surprisingly, we also find that many proteins adopt an alternative conformation. Using Fet3p as an example, we find that the temperature-dependent conformational difference is accompanied by distinct thermostability, subcellular localization, and, importantly, cellular functions. We postulate that, in addition to the known mechanisms of adaptation, conformational plasticity allows some polypeptides to acquire new biophysical properties and functions when environmental change endures.

VL - 81 IS - 16 ER -