TY - JOUR T1 - Nanoarchitectonics for Free-Standing Polyelectrolyte Multilayers Films: Exploring the Flipped Surfaces JF - ChemNanoMat Y1 - 2022 A1 - Suman Pahal A1 - Rakshith Boranna A1 - Abinash Tripathy A1 - Venkanagouda S. Goudar A1 - Vyshnavi T. Veetil A1 - Rajendra Kurapati A1 - Gurusiddappa R. Prashanth A1 - Praveen K. Vemula UR - https://doi.org/10.1002%2Fcnma.202200462 ER - TY - JOUR T1 - Exploring membrane permeability of Tomatidine to enhance lipid mediated nucleic acid transfections JF - Biochimica et Biophysica Acta (BBA) - Biomembranes Y1 - 2018 A1 - Vignesh K. Rangasami A1 - Brijesh Lohchania A1 - Chandrashekhar Voshavar A1 - Harikrishna R. Rachamalla A1 - Rajkumar Banerjee A1 - Ashish Dhayani A1 - Saravanabhavan Thangavel A1 - Praveen K. Vemula A1 - Srujan Marepally KW - Endocytosis AB -

Abstract Intracellular delivery of nucleic acids is one of the critical steps in the transfections. Prior findings demonstrated various strategies including membrane fusion, endosomal escape for the efficient cytoplasmic delivery. In our continuing efforts to improve the nucleic acids transfections, we harnessed cell permeable properties of Tomatidine (T), a steroidal alkaloid abundantly found in green tomatoes for maximizing intracellular delivery of lipoplexes. We doped Tomatidine into liposomes of cationic lipid with amide linker (A) from our lipid library. Six liposomal formulations (AT) of Lipid A (1 mM) with varying concentrations of Tomatidine (0–1 mM) were prepared and evaluated for their transfection efficacies. Owing to its signature characteristic of cell membrane permeability, Tomatidine modulated endocytosis process, enhanced the intracellular delivery of the lipoplexes, and in turn increased the transfection efficacy of cationic liposomes. Our findings provide ‘proof of concept’ for enhancing transfections in gene delivery applications with Tomatidine in cationic liposomal formulations. These findings can be further applied in lipid mediated gene therapy and drug delivery applications.

UR - https://www.sciencedirect.com/science/article/pii/S0005273618301780 ER -