TY - JOUR T1 - Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line. JF - Stem Cell Res Y1 - 2019 A1 - Schmid, Benjamin A1 - Prehn, Kennie R A1 - Nimsanor, Natakarn A1 - Garcia, Blanca Irene Aldana A1 - Poulsen, Ulla A1 - Jørring, Ida A1 - Rasmussen, Mikkel A A1 - Clausen, Christian A1 - Mau-Holzmann, Ulrike A A1 - Ramakrishna, Sarayu A1 - Muddashetty, Ravi A1 - Steeg, Rachel A1 - Bruce, Kevin A1 - Mackintosh, Peter A1 - Ebneth, Andreas A1 - Holst, Bjørn A1 - Cabrera-Socorro, Alfredo AB -

Alzheimer's disease (AD) is the most frequent neurodegenerative disease amongst the elderly. The SNPs rs429358 and rs7412 in the APOE gene are the most common risk factor for sporadic AD, and there are three different alleles commonly referred to as APOE-ε2, APOE-ε3 and APOE-ε4. Induced pluripotent stem cells (iPSCs) hold great promise to model AD as such cells can be differentiated in vitro to the required cell type. Here we report the use of CRISPR/Cas9 technology employed on iPSCs from a healthy individual with an APOE-ε3/ε4 genotype to obtain isogenic APOE-ε2/ε2, APOE-ε3/ε3, APOE-ε4/ε4 lines as well as an APOE-knock-out line.

VL - 34 ER -