TY - JOUR T1 - Altered steady state and activity-dependent de novo protein expression in fragile X syndrome. JF - Nat Commun Y1 - 2019 A1 - Bowling, Heather A1 - Bhattacharya, Aditi A1 - Zhang, Guoan A1 - Alam, Danyal A1 - Lebowitz, Joseph Z A1 - Bohm-Levine, Nathaniel A1 - Lin, Derek A1 - Singha, Priyangvada A1 - Mamcarz, Maggie A1 - Puckett, Rosemary A1 - Zhou, Lili A1 - Aryal, Sameer A1 - Sharp, Kevin A1 - Kirshenbaum, Kent A1 - Berry-Kravis, Elizabeth A1 - Neubert, Thomas A A1 - Klann, Eric AB -

Whether fragile X mental retardation protein (FMRP) target mRNAs and neuronal activity contributing to elevated basal neuronal protein synthesis in fragile X syndrome (FXS) is unclear. Our proteomic experiments reveal that the de novo translational profile in FXS model mice is altered at steady state and in response to metabotropic glutamate receptor (mGluR) stimulation, but the proteins expressed differ under these conditions. Several altered proteins, including Hexokinase 1 and Ras, also are expressed in the blood of FXS model mice and pharmacological treatments previously reported to ameliorate phenotypes modify their abundance in blood. In addition, plasma levels of Hexokinase 1 and Ras differ between FXS patients and healthy volunteers. Our data suggest that brain-based de novo proteomics in FXS model mice can be used to find altered expression of proteins in blood that could serve as disease-state biomarkers in individuals with FXS.

VL - 10 IS - 1 ER -