%0 Journal Article %J Nat Commun %D 2022 %T Intermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life. %A Pandey, Subhashini %A Mahato, Manohar %A Srinath, Preethem %A Bhutani, Utkarsh %A Goap, Tanu Jain %A Ravipati, Priusha %A Vemula, Praveen Kumar %K Acridines %K Animals %K Drug-Related Side Effects and Adverse Reactions %K Erythrocytes %K Humans %K Mice %K Nanofibers %K Research Personnel %X

Transfusion of healthy red blood cells (RBCs) is a lifesaving process. However, upon storing RBCs, a wide range of damage-associate molecular patterns (DAMPs), such as cell-free DNA, nucleosomes, free-hemoglobin, and poly-unsaturated-fatty-acids are generated. DAMPs can further damage RBCs; thus, the quality of stored RBCs declines during the storage and limits their shelf-life. Since these DAMPs consist of either positive or negative charged species, we developed taurine and acridine containing electrospun-nanofibrous-sheets (Tau-AcrNFS), featuring anionic, cationic charges and an DNA intercalating group on their surfaces. We show that Tau-AcrNFS are efficient in scavenging DAMPs from stored human and mice RBCs ex vivo. We find that intermittent scavenging of DAMPs by Tau-AcrNFS during the storage reduces the loss of RBC membrane integrity and reduces discocytes-to-spheroechinocytes transformation in stored-old-RBCs. We perform RBC-transfusion studies in mice to reveal that intermittent removal of DAMPs enhances the quality of stored-old-RBCs equivalent to freshly collected RBCs, and increases their shelf-life by ~22%. Such prophylactic technology may lead to the development of novel blood bags or medical device, and may therefore impact healthcare by reducing transfusion-related adverse effects.

%B Nat Commun %V 13 %P 7394 %8 2022 Dec 01 %G eng %N 1 %R 10.1038/s41467-022-35269-3 %0 Journal Article %J Eur J Pharm Biopharm %D 2021 %T Microneedles for Extended Transdermal Therapeutics: A Route to Advanced Healthcare. %A Pahal, Suman %A Badnikar, Kedar %A Ghate, Vivek %A Bhutani, Utkarsh %A Nayak, Mangalore Manjunatha %A Subramanyam, Dinesh Narasimhaiah %A Vemula, Praveen Kumar %X

Sustained release of drugs over a pre-determined period is required to maintain an effective therapeutic dose for variety of drug delivery applications. Transdermal devices such as polymeric microneedle patches and other microneedle-based devices have been utilized for sustained release of their payload. Swift clearing of drugs can be prevented either by designing a slow-degrading polymeric matrix or by providing physiochemical triggers to different microneedle-based devices for on-demand release. These long-acting transdermal devices prevent the burst release of drugs. This review highlights the recent advances of microneedle-based devices for sustained release of vaccines, hormones, and antiretrovirals with their prospective safe clinical translation.

%B Eur J Pharm Biopharm %V 159 %P 151-169 %8 2021 Feb %G eng %R 10.1016/j.ejpb.2020.12.020