InStem Mass spec facility for Qualitiative and Quantitative charactetization of Biomolecules 

Zeno TOF 7600 - Zeno TOF 7600 is coupled high flow UPLC for analysis of metabolite, Protein, Peptide and Lipids. The 7600 Zeno TOF having the power of Zeno trap in combination with the binary fragmention mechnism i.e. Collision induced dissociaton and  EAD ( Electron activated dissociation) is very useful for the characterization of biomolecules. The high resolution of the analyzer is well suited for the characterization of biosimilars, Biobetter and intact proteins.Due to zeno trapping of the ion the sensitivity of this platfrom is enhanced multiple fold and dynamic range for the quantitation has also got improved significantly.

Following Mass spectrometry Platfrom is present in the BLiSc cluster institutes.

Thermo Q-Exactive

5600 Triple TOF with Capillary LC and Conventional UPLC

TSQ Vantage-Agilent 1290 UHPLC (LC-MS)

Thermo Orbitrap Fusion with Nano LC

Sciex QTRAP 6500 MS/MS with Water UPLC

Sciex QTRAP 5500 MS/MS with UPLC

Thermo LTQ XL with LC

Thermo Fleet

GCMS single Quad

GCMS Triple Quad

 

Instruments for Proteomics / Protein Characterization

 

   
Thermo Q-Exactive - The combination of LC separation, high resolution MS and MS2 analysis should provide unambiguous and precise characterization of different classes of lipids in biological samples. Q-exactive MS with its mass resolution of 140,000 at 400 m/z and mass accuracy of 1-2 ppm is optimized for lipidomics and metabolomics experiments.  HCD fragmentation using stepped normalised collision energies is an excellent tool for identification and quantitation of different classes of lipids. Sciex QTRAP 6500 MS/MS - One of the best is class triple-quadrupole MS instrument for the targeted quantitative analysis with MS3 fragemntation that enable for identification as well. It also equipped with the differnetial ion mobility technology.
   
   
Agilent nano HPLC - This LC system with flow rate ranges from 100 nl/min to 1ul/min is suitable for high sensitivity MS detection and 2 dimensional separation. Advion Triversa NanoMate Robot - Uses a chip based technology for stable sample infusion at nano litre flow rate.
   
   
TSQ Vantage-Agilent 1290 UHPLC (LC-MS) - The Thermo Scientific TSQ Vantage uses precision hyperbolic quadrupoles, which provide the best transmission and peak shape, the detection technology and ion source results. It is the most sensitive triple quadrupole instrument. Agilent’s 1290 infinity series UHPLC system interfaces to the mass spectrometer for the automated LC-MS/MRM analysis.
Thermo LTQ Orbitrap Discovery - This MS with its mass resolution of 30,000 at 400 m/z and mass accuracy of 1-2 ppm is optimized for high throughput proteomics experiments. It has two fragmentation techniques, CID and PQD, associated with and is an excellent tool for labeled and label-free quantitation.