Assessment of the inherent chondrogenic potential of human articular cartilage-derived chondroprogenitors in pellet culture using a novel whole pellet processing approach.
Title | Assessment of the inherent chondrogenic potential of human articular cartilage-derived chondroprogenitors in pellet culture using a novel whole pellet processing approach. |
Publication Type | Journal Article |
Year of Publication | 2022 |
Authors | Johnson NNaveen, Amirtham SManickam, B Rani S, Sathishkumar S, Rebekah G, Vinod E |
Journal | J Orthop |
Volume | 31 |
Pagination | 45-51 |
Date Published | 2022 May-Jun |
ISSN | 0972-978X |
Abstract | Purpose: Cartilage-derived chondroprogenitors have been reported to possess the biological potential for cartilage repair. However, its inherent chondrogenic potential in pellet culture needs evaluation. In-vitro cartilage regeneration models based on pellet cultures have been employed to evaluate the chondrogenic potential of stem cells. Evaluation of the degree of differentiation routinely involves paraffin embedding, sectioning, and immunohistochemical staining of the pellet. However, since chondrogenic differentiation is commonly non-uniform, processing random sections could lead to inaccurate conclusions. The study aimed at assessing the inherent lineage bias of chondroprogenitors with and without chondrogenic induction, using a novel whole pellet processing technique. Methods: Human chondroprogenitors (n=3) were evaluated for MSC markers and processed in pellet cultures either with stromal medium (uninduced) or chondrogenic differentiation medium (induced) for 28 days. The whole pellets and the conventional paraffin-embedded sectioned pellets were subjected to Collagen type II immunostaining and assessed using confocal laser microscopy. The staining intensities of the whole pellet were compared to the paraffin sections and revalidated using qRT-PCR for COL2A1 expression. Results: Uninduced and induced pellets displayed Collagen type II in all the layers with comparable fluorescence intensities. COL2A1 expression in both pellets was comparable to confocal results. The study demonstrated that uninduced chondroprogenitors in pellet culture possess promising inherent chondrogenic potential. Confocal imaging of whole pellets displayed different degrees of chondrogenic differentiation in the entire pellet, thus its probable in-vivo behavior. Conclusion: The novel approach presented in this study could serve as an efficient in-vitro alternative for understanding translational application for cartilage repair. |
DOI | 10.1016/j.jor.2022.03.007 |
Alternate Journal | J Orthop |
PubMed ID | 35368732 |
PubMed Central ID | PMC8967706 |
Comments
Very useful for me.
Contact https://penaltykickonline.com
Thanks for sharing this very useful resource, I'll be sure to keep it handy for when I need it! I will also share it with the people from my favorite HVAC Company in Gainesville, Florida https://www.daytonheatandair.com/about
Cartilage-derived chondroprogenitors have been shown to have the biological potential to heal cartilage. However, its inherent chondrogenic potential in pellet culture must be investigated. To assess the chondrogenic capability of stem cells, in-vitro cartilage regeneration models based on pellet cultures were used. https://streetfighterduel.io/
I really value the advice you've given me and the insights you've shared. I want to get as knowledgeable as possible about it so that I may use it to its full potential. https://redactleunlimited.com
I appreciate the details and recommendations you have offered. For further information, I'll try to better comprehend it. Visit https://geometrydashmeltdown.com
This are some great and useful resourses, thank you for sharing them with us, I will share them with https://www.concretecontractorsfargond.com/driveway-replacement-fargo
I am genuinely thankful to the holder of this web page who has shared this wonderful paragraph at at this place https://iogamesio.org/lolshotio
The potency of chondrogen and chondroprogenitors depends on many factors, including the method of culture, their biological characteristics, and the intended application. https://aarouteplanner.io
I really appreciate the website owner for such this nice articles at this place. https://baldisbasics.io
I have read a lot of other blogs, but the one you have written is the one that has convinced me. I hope that you will continue to write significant blogs in the future so that you can share them with your audience. https://globle-wordle.com
Current cartilage repair research is centered on the utilization of soluble substances generated by cells during expansion as stand-alone therapy or as conditioned medium (CM) additives to enhance cellular phenotype for in-vivo transplantation. https://uno-online.co/
I greatly appreciate the insights and recommendations you have provided me. My intention is to acquire comprehensive knowledge about it in order to utilize it proficiently.
https://www.akm941roofing.com/holmesbeach.html
https://www.floridalocalroofers.com/northport.html
After reviewing numerous blogs, I have found your written piece to be the most persuasive. I look forward to your future endeavours in producing impactful blog content for your audience.
Donkey Kong required players to jump between gaps and jump over obstacles or approach enemies, setting the template for the future of platforming. https://donkey-kong.io
The information in the article impressed me very much. They are all very good information and helped me a lot in my research on this new topic. https://retrobowlmod.com
Emoji may be quickly copied and pasted into Twitter, Gmail, or Facebook from your Android or iPhone device. Utilize our searchable online emoji keyboard to locate emoji codes or obtain them in a flash! https://emojihub.org
More scientific discoveries leading to expanded human development knowledge! Such a beautiful day for everyone. Well, if you're looking to make your house beautiful just by restoring your roof, go to our website now! https://www.cairnsroofingservices.com/contact/
In pellet culture, https://fnfgame.io chondroprogenitor cells are aggregated and cultured in a three-dimensional environment, mimicking the conditions found in native cartilage tissue. This system provides a suitable environment for the cells to undergo chondrogenesis, the process by which they differentiate into mature chondrocytes and produce cartilage matrix components.
The novel whole pellet processing approach likely refers to a specific https://wordle2.io method used to analyze the chondrogenic potential of the cells within the pellets.
Paraffin embedding, sectioning, and immunohistochemical staining of the pellet are frequently used to assess the degree of differentiation. Processing arbitrary sections could result in incorrect conclusions, though, as chondrogenic differentiation is frequently non-uniform. See: https://plumberswestauckland.co.nz
Emoji allows you to quickly copy and paste all the emoji characters into your preferred editor. https://emojihub.org
The study aimed at assessing the inherent lineage bias of chondroprogenitors with and without chondrogenic induction, using a novel whole pellet processing technique.
https://www.dallasdrywallsolutions.com/drywall-contractor-dallas-texas
-
Amanda, Fri, 09/01/2023 - 23:50:
This assessment is very accurate and https://g.co/kgs/jPBZsU also approves.
. Evaluation of the degree of differentiation routinely involves paraffin embedding, sectioning, and immunohistochemical staining of the pellet. See: https://drywalldallas.com/drywall-repair-dallas-texas/
I love the way you write. Your blog always keeps me engaged and eager to read more. https://www.localcashpropertybuyers.com/killeen-tx.html
The whole pellets and the conventional paraffin-embedded sectioned pellets were subjected to Collagen type II immunostaining and assessed using confocal laser microscopy.
See: https://www.flashpartyphotobooth.com/conferences
Interesting information, but I think it would be really useful if you could add a photo and information where you could process the data and change https://depositphotos.com/upscaler.html upscale picture the photo extension. Therefore, it is important to carry out the correct photo processing in order not to lose quality
Thanks for sharing this information, I will be sure to share it with the people from my favorite roofings inspection company https://worthmannroofing.com/roof-inspection/
The objective of this systematic review was to identify and collate information pertaining to hyaline cartilage–derived chondroprogenitors.
I often read what you write because I find it both interesting and helpful https://iogamesfree.io
I appreciate how you've managed to make https://subwaysurfersgame.io complex concepts easily understandable through your writing.
However, since chondrogenic differentiation is commonly non-uniform, processing random sections could lead to inaccurate conclusions. See: https://www.royalroofcontractorsllc.net/residential-roofing
-
afsd, Thu, 08/17/2023 - 17:16:
I think this post is very helpful and full of useful information Very nice blog , give feedback https://linkfly.to/publixsurvey-online
Evaluation of the degree of differentiation routinely involves paraffin embedding, sectioning, and immunohistochemical staining of the pellet. See: https://www.aldodrywallcontractor.com/residential-drywall
Evaluation of the degree of differentiation routinely involves paraffin embedding, sectioning, and immunohistochemical staining of the pellet. https://www.miamifoundationrepair.com/basement-waterproofing-miami-flori...
What a deep and well written study. Thanks for sharing https://sc-empresadeclimatizacion.cl
Their ability to convey complex ideas with clarity and conciseness not only made the content easily digestible but also highlighted the depth of their knowledge and expertise on the subject.
The depth of wisdom and unique perspectives presented in this article made it an absolute gem that I will undoubtedly revisit time and again. I cannot emphasize enough how much I appreciated the author's insights, which brought a fresh perspective to the subject and allowed me to see it from a whole new angle.
Science is becoming stronger and stronger than ever, I believe that with this application you will be able to cure many patients who are facing this disease.https://wordle-nyt.org/
The study presents a novel method to evaluate chondrogenic potential in human articular cartilage-derived chondroprogenitors using a comprehensive pellet culture approach. This innovative technique offers valuable insights into cartilage regeneration and could enhance our understanding of musculoskeletal therapies, potentially advancing treatments for joint-related conditions https://supertoiletrentalsatlanta.com/services/restroom-trailer-rental-a...
The study investigates the inherent chondrogenic potential of human articular cartilage-derived chondroprogenitors using a unique pellet culture method. Chondroprogenitors are cells capable of becoming chondrocytes, which form cartilage tissue. The research employs a novel whole pellet processing approach, likely involving innovative techniques for maintaining pellet integrity and analyzing them comprehensively. Check this website https://games.lol/fate-grand-order/.
By cultivating these cells in three-dimensional pellet structures, the study aims to mimic a more natural cellular environment for evaluating their differentiation into chondrocytes. This research holds significance for understanding the regenerative capabilities of these cells and their potential applications in treating cartilage-related conditions. You can look it up here https://answersrepublic.com/health/why-is-it-so-hard-to-see-an-endocrino....
This systematic review's goal was to locate and compile data on chondroprogenitors produced from hyaline cartilage. https://dinosaurgames.io
Do you mind if I quote a couple of your articles as long as I provide credit and sources back to your website? https://www.parratreeremoval.com.au/
My blog is in the exact same area of interest as yours and my users would genuinely benefit from a lot of the information you present here. https://www.crosscarendis.com.au/
Thank you for posting like this keep it up! https://www.google.com/maps/place/?q=place_id:ChIJ48xZLbp1cVMRmkuG0vDqeYo
Cartilage-derived chondroprogenitors have been shown to have the biological potential to heal cartilage. However, its inherent chondrogenic potential in pellet culture must be investigated. To assess the chondrogenic capability of stem cells, in-vitro cartilage <a href="https://theminicrossword.com">Mini Crossword</a> regeneration models based on pellet cultures were used.
Pages
- 1
- 2
The insights and recommendations you've given me are greatly appreciated. I intend to learn all I can about it so that I can use it effectively.
I'm glad to hear that you found the insights and recommendations helpful! Learning and understanding as much as you can will definitely empower you to use it effectively. If you have any further questions or need more guidance along the way, feel free to reach out. Best of luck on your journey of mastering it! https://www.grandrapidsductcleaning.co/services/air-filter-replacement-g...