Characterization of new variant human ES line VH9 hESC (INSTEMe001-a): a tool for human stem cell and cancer research.
|Title||Characterization of new variant human ES line VH9 hESC (INSTEMe001-a): a tool for human stem cell and cancer research.|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Arasala RRao, Jayaram M, Chattai J, Kumarasamy T, Sambasivan R, Rampalli S|
|Journal||Stem Cell Res|
|Date Published||2019 May|
Human pluripotent stem cells (hPSCs) acquire changes at the genomic level upon proliferation and differentiation (Peterson and Loring, 2014). Studies from International Stem Cell Initiative and independent laboratories identified a copy number variant (CNV) in hES cell lines displaying a normal karyotype, which provided a selective advantage to hES cells in culture. In our laboratory we have identified variant H9-hESC (derived from H9-hESC) with normal karyotype, pluripotency expression, differentiation profile but with altered traits of high cell survival and low E-CADHERIN expression.
|Alternate Journal||Stem Cell Res|