TitleNanobody derived using a peptide epitope from the spike protein receptor-binding motif inhibits entry of SARS-CoV-2 variants.
Publication TypeJournal Article
Year of Publication2022
AuthorsMendon N, Ganie RA, Kesarwani S, Dileep D, Sasi S, Lama P, Chandra A, Sirajuddin M
JournalJ Biol Chem
Volume299
Issue1
Pagination102732
Date Published2022 Nov 22
ISSN1083-351X
Abstract

The emergence of new escape mutants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has escalated its penetration among the human population and has reinstated its status as a global pandemic. Therefore, developing effective antiviral therapy against emerging SARS-CoV variants and other viruses in a short period becomes essential. Blocking SARS-CoV-2 entry into human host cells by disrupting the spike glycoprotein-angiotensin-converting enzyme 2 interaction has already been exploited for vaccine development and monoclonal antibody therapy. Unlike the previous reports, our study used a nine-amino acid peptide from the receptor-binding motif of the spike protein as an epitope. We report the identification of an efficacious nanobody N1.2 that blocks the entry of pseudovirus-containing SARS-CoV-2 spike as the surface glycoprotein. Moreover, using mCherry fluorescence-based reporter assay, we observe a more potent neutralizing effect against both the hCoV19 (Wuhan/WIV04/2019) and the Omicron (BA.1) pseudotyped spike virus with a bivalent version of the N1.2 nanobody. In summary, our study presents a rapid and efficient methodology to use peptide sequences from a protein-receptor interaction interface as epitopes for screening nanobodies against potential pathogenic targets. We propose that this approach can also be widely extended to target other viruses and pathogens in the future.

DOI10.1016/j.jbc.2022.102732
Alternate JournalJ Biol Chem
PubMed ID36423687
PubMed Central IDPMC9678391

Comments

  • recitepleasing, Thu, 06/15/2023 - 03:42:

    New SARS-CoV-2 escape mutations have increased the virus's spread across the human population and restored the pandemic status of severe acute respiratory syndrome. Therefore, it is crucial to rapidly develop effective antiviral treatment against new SARS-CoV variants and other viruses. https://dordle.io

  • theodoreevans, Tue, 08/01/2023 - 03:30:

    I think this post is very helpful and full of useful information. So, I'd like to thank you for all the hard work you put into writing this article https://fngames.io

  • viettrau, Fri, 08/04/2023 - 02:10:

    The world has been through a pandemic for several years and has damaged people's health and economy, fortunately we quickly found a vaccine to solve it and everything is back to normal. often. https://wordlewordle.org/revertle

  • Nanasi, Mon, 08/28/2023 - 09:43:

    Collection of word games. You can comfortably study while playing on word games. Surely you will feel satisfied about the games it offers https://wordgames.gg

  • moto x3m, Sat, 09/09/2023 - 03:31:

    This is exactly what I was looking for, please post more posts on this topic
    https://motox3mgame.io/

Leave a Reply

Plain text

  • No HTML tags allowed.
  • Web page addresses and e-mail addresses turn into links automatically.
  • Lines and paragraphs break automatically.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
1 + 3 =

Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.