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Next Generation Genomics Facility - Services

(a) Experiment Design- Providing advice on NGS experimental design, sequencing depth etc.

(b) DNA/RNA shearing- using Covaris-S220 Focused-ultrasonicator

(c) Library Preparation- User training and support in library preparation using various protocols (DNA, mRNA, small RNA, ChIP, Amplicon, Degradome sequencing, Transposon Directed Insertion Sequencing (TraDIS) , Metagenomics) for Illumina sequencing

(d) Quality and quantity assessments of DNA/RNA- using Bioanalyzer, Qubit and KAPA Library Quant kit

(e) Sequencing- (a) Sanger sequencing-ABI3730 (b) Illumina Hiseq 2500 (c) Illumina Miseq (d) MinIon (Nanopore)

(f) Droplet Digital PCR (ddPCR) Services- Absolute Quantification , Copy Number Variation and Rare Event Detection