TitleIntermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life.
Publication TypeJournal Article
Year of Publication2022
AuthorsPandey S, Mahato M, Srinath P, Bhutani U, Goap TJain, Ravipati P, Vemula PKumar
JournalNat Commun
Volume13
Issue1
Pagination7394
Date Published2022 Dec 01
ISSN2041-1723
KeywordsAcridines, Animals, Drug-Related Side Effects and Adverse Reactions, Erythrocytes, Humans, Mice, Nanofibers, Research Personnel
Abstract

Transfusion of healthy red blood cells (RBCs) is a lifesaving process. However, upon storing RBCs, a wide range of damage-associate molecular patterns (DAMPs), such as cell-free DNA, nucleosomes, free-hemoglobin, and poly-unsaturated-fatty-acids are generated. DAMPs can further damage RBCs; thus, the quality of stored RBCs declines during the storage and limits their shelf-life. Since these DAMPs consist of either positive or negative charged species, we developed taurine and acridine containing electrospun-nanofibrous-sheets (Tau-AcrNFS), featuring anionic, cationic charges and an DNA intercalating group on their surfaces. We show that Tau-AcrNFS are efficient in scavenging DAMPs from stored human and mice RBCs ex vivo. We find that intermittent scavenging of DAMPs by Tau-AcrNFS during the storage reduces the loss of RBC membrane integrity and reduces discocytes-to-spheroechinocytes transformation in stored-old-RBCs. We perform RBC-transfusion studies in mice to reveal that intermittent removal of DAMPs enhances the quality of stored-old-RBCs equivalent to freshly collected RBCs, and increases their shelf-life by ~22%. Such prophylactic technology may lead to the development of novel blood bags or medical device, and may therefore impact healthcare by reducing transfusion-related adverse effects.

DOI10.1038/s41467-022-35269-3
Alternate JournalNat Commun
PubMed ID36450757
PubMed Central IDPMC9712616

Comments

  • Annata Even, Wed, 07/26/2023 - 16:08:

    When RBCs are stored, a variety of damage-associated molecular patterns (DAMPs) such as cell-free DNA, nucleosomes, free-hemoglobin, and poly-unsaturated fatty acids are produced. DAMPs can further damage RBCs, causing the quality of stored https://lego2kdrive.io/ RBCs to deteriorate and limiting their shelf-life.

  • quarteringzeal, Thu, 07/27/2023 - 04:39:

    Even while Alabama isn't well recognized as a prime location for outdoor activities, that doesn't mean the National Park Service doesn't have anything to offer https://driving-directions.co Just six blocks later, the protestors were ambushed by local authorities. A second march to the bridge was led by Martin Luther King Jr. two days later.

  • motivationrun, Tue, 10/10/2023 - 07:49:

    Such prophylactic technology may lead to the development of novel blood bags or medical device, and may therefore impact healthcare by reducing transfusion-related adverse effects. https://slope-unblocked.co

Leave a Reply

Plain text

  • No HTML tags allowed.
  • Web page addresses and e-mail addresses turn into links automatically.
  • Lines and paragraphs break automatically.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
3 + 1 =

Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.